Midazinski homolog MDN1 (kvasac) jest protein koji je kod ljudi kodiran genom MDN. Midazin je član porodice AAAATPaza.
Aminokiselinska sekvenca Dužina polipeptidnog lanca je 5.596 aminokiselina, а molekulska težina 632.820 Da.
1020304050 MEHFLLEVAAAPLRLIAAKNEKSRSELGRFLAKQVWTPQDRQCVLSTLAQ LLLDKDCTVLVGRQLRPLLLDLLERNAEAIKAGGQINHDLHERLCVSMSK LIGNHPDVLPFALRYFKDTSPVFQRLFLESSDANPVRYGRRRMKLRDLME AAFKFLQQEQSVFRELWDWSVCVPLLRSHDTLVRWYTANCLALVTCMNEE HKLSFLKKIFNSDELIHFRLRLLEEAQLQDLEKALVLANPEVSLWRKQKE LQYLQGHLVSSDLSPRVTAVCGVVLPGQLPAPGELGGNRSSSREQELALR SYVLVESVCKSLQTLAMAVASQNAVLLEGPIGCGKTSLVEYLAAVTGRTK PPQLLKVQLGDQTDSKMLLGMYRCTDVPGEFVWQPGTLTQAATMGHWILL EDIDYAPLDVVSVLIPLLENGELLIPGRGDCLKVAPGFQFFATRRLLSCG GNWYRPLNSHATLLDKYWTKIHLDNLDKRELNEVLQSRYPSLLAVVDHLL DIYIQLTGEKHHSWSDSSVGCEQAPEEVSEARRENKRPTLEGRELSLRDL LNWCNRIAHSFDSSSLSASLNIFQEALDCFTAMLSEHTSKLKMAEVIGSK LNISRKKAEFFCQLYKPEIVINELDLQVGRVRLLRKQSEAVHLQREKFTF AATRPSSVLIEQLAVCVSKGEPVLLVGETGTGKTSTIQYLAHITGHRLRV VNMNQQSDTADLLGGYKPVDHKLIWLPLREAFEELFAQTFSKKQNFTFLG HIQTCYRQKRWHDLLRLMQHVHKSAVNKDGKDSETGLLIKEKWEAFGLRL NHAQQQMKMTENTLLFAFVEGTLAQAVKKGEWILLDEINLAAPEILECLS GLLEGSSGSLVLLDRGDTEPLVRHPDFRLFACMNPATDVGKRNLPPGIRN RFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGT KLVDGTGHRPHYSLRTLCRALRFAASNPCGNIQRSLYEGFCLGFLTQLDR ASHPIVQKLICQHIVPGNVKSLLKQPIPEPKGGRLIQVEGYWIAVGDKEP TIDETYILTSSVKLNLRDIVRVVSAGTYPVLIQGETSVGKTSLIQWLAAA TGNHCVRINNHEHTDIQEYIGCYTSDSSGKLVFKEGVLIDAMRKGYWIIL DELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHPRFMLFATQNPPGL YGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVM LDLQSYRRSSSVFAGKQGFITLRDLFRWAERYRLAEPTEKEYDWLQHLAN DGYMLLAGRVRKQEEIDVIQEVLEKHFKKKLCPQSLFSKENVLKLLGKLS TQISTLECNFGHIVWTEGMRRLAMLVGRALEFGEPVLLVGDTGCGKTTIC QVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEW HDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPE DKDSEIELLTAGKKFRILATMNPGGDFGKKELSPALRNRFTEIWCPQSTS REDLIQIISHNLRPGLCLGRIDPKGSDIPEVMLDFIDWLTHQEFGRKCVV SIRDILSWVNFMNKMGEEAALKRPEIISTVTSFVHAACLVYIDGIGSGVT SSGFGTALLARKECLKFLIKRLAKIVRLTEYQKNELKIYDRMKAKEFTGI DNLWGIHPFFIPRGPVLHRNNIADYALSAGTTAMNAQRLLRATKLKKPIL LEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKG GEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPE LGMSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVID MEFIASTLFPAIEKNIVKKMVAFNNQIDHEVTVEKKWGQKGGPWEFNLRD LFRWCQLMLVDQSPGCYDPGQHVFLVYGERMRTEEDKKKVIAVFKDVFGS NSNPYMGTRLFRITPYDVQLGYSVLSRGSCVPHPSRHPLLLLHQSFQPLE SIMKCVQMSWMVILVGPASVGKTSLVQLLAHLTGHTLKIMAMNSAMDTTE LLGGFEQVDLIRPWRRLLEKVEGTVRALLRDSLLISADDAEVVLRAWSHF LLTYKPKCLGEGGKAITMEIVNKLEAVLLLMQRLNNKINSYCKAEFAKLV EEFRSFGVKLTQLASGHSHGTFEWVDSMLVQALKSGDWLLMDNVNFCNPS VLDRLNALLEPGGVLTISERGMIDGSTPTITPNPNFRLFLSMDPVHGDIS RAMRNRGLEIYISGEGDASTPDNLDLKVLLHSLGLVGNSVCDILLALHTE TRSTVVGSPTSSVSTLIQTAILIVQYLQRGLSLDRAFSEACWEVYVCSQH SPANRKLVQALLEKHVSSLRAHETWGDSILGMGLWPDSVPSALFATEDSH LSTVRRDGQILVYCLNRMSMKTSSWTRSQPFTLQDLEKIMQSPSPENLKF NAVEVNTYWIDEPDVLVMAVKLLIERATNQDWMLRVKWLYHLAKNIPQGL ESIQIHLEASAASLRNFYSHSLSGAVSNVFKILQPNTTDEFVIPLDPRWN MQALDMIRNLMDFDPQTDQPDQLFALLESAANKTIIYLDREKRVFTEANL VSVGSKKLRESVLRMSFEFHQDPESYHTLPHEIVVNLAAFFELCDALVLL WVQSSQGMVSDASANEILGSLRWRDRFWTVADTVKVDAPGLALLALHWHW VLKHLVHQIPRLLMNYEDKYYKEVQTVSEHIQNCLGSQTGGFAGIKKLQK FLGRPFPFKDKLVVECFSQLKVLNKVLAIREQMSALGESGWQEDINRLQV VASQWTLKKSLLQAWGLILRANILEDVSLDELKNFVHAQCLELKAKGLSL GFLEKKHDEASSLSHPDLTSVIHLTRSVQLWPAMEYLAMLWRYKVTADFM AQACLRRCSKNQQPQINEEISHLISFCLYHTPVTPQELRDLWSLLHHQKV SPEEITSLWSELFNSMFMSFWSSTVTTNPEYWLMWNPLPGMQQREAPKSV LDSTLKGPGNLNRPIFSKCCFEVLTSSWRASPWDVSGLPILSSSHVTLGE WVERTQQLQDISSMLWTNMAISSVAEFRRTDSQLQGQVLFRHLAGLAELL PESRRQEYMQNCEQLLLGSSQAFQHVGQTLGDMAGQEVLPKELLCQLLTS LHHFVGEGESKRSLPEPAQRGSLWVSLGLLQIQTWLPQARFDPAVKREYK LNYVKEELHQLQCEWKTRNLSSQLQTGRDLEDEVVVSYSHPHVRLLRQRM DRLDNLTCHLLKKQAFRPQLPAYESLVQEIHHYVTSIAKAPAVQDLLTRL LQALHIDGPRSAQVAQSLLKEEASWQQSHHQFRKRLSEEYTFYPDAVSPL QASILQLQHGMRLVASELHTSLHSSMVGADRLGTLATALLAFPSVGPTFP TYYAHADTLCSVKSEEVLRGLGKLILKRSGGKELEGKGQKACPTREQLLM NALLYLRSHVLCKGELDQRALQLFRHVCQEIISEWDEQERIAQEKAEQES GLYRYRSRNSRTALSEEEEEEREFRKQFPLHEKDFADILVQPTLEENKGT SDGQEEEAGTNPALLSQNSMQAVMLIHQQLCLNFARSLWYQQTLPPHEAK HYLSLFLSCYQTGASLVTHFYPLMGVELNDRLLGSQLLACTLSHNTLFGE APSDLMVKPDGPYDFYQHPNVPEARQCQPVLQGFSEAVSHLLQDWPEHPA LEQLLVVMDRIRSFPLSSPISKFLNGLEILLAKAQDWEENASRALSLRKH LDLISQMIIRWRKLELNCWSMSLDNTMKRHTEKSTKHWFSIYQMLEKHMQ EQTEEQEDDKQMTLMLLVSTLQAFIEGSSLGEFHVRLQMLLVFHCHVLLM PQVEGKDSLCSVLWNLYHYYKQFFDRVQAKIVELRSPLEKELKEFVKISK WNDVSFWSIKQSVEKTHRTLFKFMKKFEAVLSEPCRSSLVESDKEEQPDF LPRPTDGAASELSSIQNLNRALRETLLAQPAAGQATIPEWCQGAAPSGLE GELLRRLPKLRKRMRKMCLTFMKESPLPRLVEGLDQFTGEVISSVSELQS LKVEPSAEKEKQRSEAKHILMQKQRALSDLFKHLAKIGLSYRKGLAWARS KNPQEMLHLHPLDLQSALSIVSSTQEADSRLLTEISSSWDGCQKYFYRSL ARHARLNAALATPAKEMGMGNVERCRGFSAHLMKMLVRQRRSLTTLSEQW IILRNLLSCVQEIHSRLMGPQAYPVAFPPQDGVQQWTERLQHLAMQCQIL LEQLSWLLQCCPSVGPAPGHGNVQVLGQPPGPCLEGPELSKGQLCGVVLD LIPSNLSYPSPIPGSQLPSGCRMRKQDHLWQQSTTRLTEMLKTIKTVKAD VDKIRQQSCETLFHSWKDFEVCSSALSCLSQVSVHLQGLESLFILPGMEV EQRDSQMALVESLEYVRGEISKAMADFTTWKTHLLTSDSQGGNQMLDEGF VEDFSEQMEIAIRAILCAIQNLEERKNEKAEENTDQASPQEDYAGFERLQ SGHLTKLLEDDFWADVSTLHVQKIISAISELLERLKSYGEDGTAAKHLFF SQSCSLLVRLVPVLSSYSDLVLFFLTMSLATHRSTAKLLSVLAQVFTELA QKGFCLPKEFMEDSAGEGATEFHDYEGGGIGEGEGMKDVSDQIGNEEQVE DTFQKGQEKDKEDPDSKSDIKGEDNAIEMSEDFDGKMHDGELEEQEEDDE KSDSEGGDLDKHMGDLNGEEADKLDERLWGDDDEEEDEEEEDNKTEETGP GMDEEDSELVAKDDNLDSGNSNKDKSQQDKKEEKEEAEADDGGQGEDKIN EQIDERDYDENEVDPYHGNQEKVPEPEALDLPDDLNLDSEDKNGGEDTDN EEGEEENPLEIKEKPEEAGHEAEERGETETDQNESQSPQEPEEGPSEDDK AEGEEEMDTGADDQDGDAAQHPEEHSEEQQQSVEEKDKEADEEGGENGPA DQGFQPQEEEEREDSDTEEQVPEALERKEHASCGQTGVENMQNTQAMELA GAAPEKEQGKEEHGSGAADANQAEGHESNFIAQLASQKHTRKNTQSFKRK PGQADNERSMGDHNERVHKRLRTVDTDSHAEQGPAQQPQAQVEDADAFEH IKQGSDAYDAQTYDVASKEQQQSAKDSGKDQEEEEIEDTLMDTEEQEEFK AADVEQLKPEEIKSGTTAPLGFDEMEVEIQTVKTEEDQDPRTDKAHKETE NEKPERSRESTIHTAHQFLMDTIFQPFLKDVNELRQELERQLEMWQPRES GNPEEEKVAAEMWQSYLILTAPLSQRLCEELRLILEPTQAAKLKGDYRTG KRLNIRKVIPYIASQFRKDKIWLRRTKPSKRQYQICLAIDDSSSMVDNHT KQLAFESLAVIGNALTLLEVGQIAVCSFGESVKLLHPFHEQFSDYSGSQI LRLCKFQQKKTKIAQFLESVANMFAAAQQLSQNISSETAQLLLVVSDGRG LFLEGKERVLAAVQAARNANIFVIFVVLDNPSSRDSILDIKVPIFKGPGE MPEIRSYMEEFPFPYYIILRDVNALPETLSDALRQWFELVTASDHP
Modelni organizmi + Fenotip Mdn1 nokaut-miša Svojstvo Fenotip Vijabilnost homozigotaNenormalanStudija recesivne letalnosti Nenormalan PlodnostNormalan Tjelesna težina Normalan Anksioznost otvorenog polja Normalan Neurološka procjena Normalan Snaga stiskaNormalan Test vruće ploče Normalan Dismorfologija Normalan Indirektna kalorimetrija Normalan Test tolerancije glukoze Normalan Slušni odgovor moždanog stabla Normalan DEXA Normalan Radiografija Normalan Tjelesna temperatura Normalan Morfologija oka Normalan Klinička hemija Normalan Plazmatski imunoglobulini Normalan Hematologija Normalan Limfociti periferne krviNormalan Težina srca Normalan Histopatologija mozga Normalan Salmonella infekcija Normalan Citrobacter infekcijaNenormalanSvi testovi i analize su prema:Mouse Resources Portal, Wellcome Trust Sanger Institute. Urađena su 24 testa na mutantnim miševima i primijećene su tri značajne abnormalnosti. Nijedan homozigotni mutantni embrion nije identificiran tokom gestacije, pa nijedan nije preživio do odvikavanja. Preostali testovi su provedeni na heterozigotnim mutantnim odraslim miševima; ženke su imale povećanu osjetljivost na bakterijsku infekciju.
Reference
Dopunska literatura
Kategorija:Mišji mutirani geni Kategorija:Geni na hromosomu 6 Kategorija:Ljudski proteini Kategorija:Proteomika