La seguente lista comprende sia le compagnie aeree che le forze aeree che utilizzano o hanno utilizzato in passato il Fokker F27:
Civili All'agosto 2006 erano ancora in servizio presso le varie compagnie aeree mondiali un totale di 164 Fokker F27 (tutte le versioni), impiegati in maggior numero da Libyan Arab Airlines (14), Merpati Nusantara Airlines (11), WDL Aviation (11) e Mountain Air Cargo (11), seguite da altre 43 in numero inferiore.Flight International, 3–9 October 2006.
Air Algérie
TAAG Angola Airlines
Air ALM
CATA Línea Aérea Líneas Aéreas del Estado (LADE)
Airlines of New South Wales Ansett Airlines Associated Airlines of Australia Department of Civil Aviation East-West Airlines (Australia) AirCruising Australia Australia Post Trans Australia Airlines
Amerer Air
Biman Bangladesh Airlines
Lloyd Aéreo Boliviano
Burma Airways Corporation Union of Burma Airways Myanma Airways
Tavaj Linhas Aéreas TAM Airlines
Gov't Quebec Norcanair Quebecair Time Air
ABA Airlines
Air Ivoire
Government of Chad
Laoag
Cubana de Aviación
Air Zaire Air Tropiques
Maersk Air Newair Airservice (Denmark) Sterling Airways
Air Sinai
Falcon Express Cargo Airlines
Air Manila Laoag International Airlines Mactan Philippine Airlines
Finnair
Air France Air Inter France Institute Geographique Securité Civil
Air Max-Gabon
FTG Air Service WDL Aviation
T.A. de la Guinee-Bissau
Aerolíneas SOSA Atlantic Airlines de Honduras
Euroceltic Airways Iona National Airways Starair
Icelandair
Elbee Airlines Indian Airlines NEPC Airlines
Merpati Nusantara Airlines
Iran Aseman Airlines Iranian government National Iranian Oil Company (NIOC)
ATI Aero Trasporti Italiani Alisarda MiniLiner
All Nippon Airways
Kenya Airways
Lesotho Airways
Libyan Arab Airlines Libyan Red Crescent
Luxair
Malaysia Airlines System Malaysia-Singapore Airlines
Royal Air Maroc
Aerocaribe Air One (Mexico) (used only for cargo) CityFlyer
DETA
F27 Friendship Association The Dutch Royal Flight NLM Cityhopper
Aeronica
Afrijet Nigeria Airways
Air Executive Norway Braathens SAFE Busy Bee Norwegian Air Shuttle
Air New Zealand Airwork (New Zealand) New Zealand Ministry of Transport (Navaids calibration flight) NZNAC
Airlift International Pakistan International Airlines
Air Panama
Air Niugini
Aero Condor
Expresso Aéreo
Oceanair
Air Anglia Air UK British Midland Channel Express Jersey European Airways Manx Airlines
Somali Airlines
Aviaco Iberia Airlines Seven Air Spantax Transeuropa
Mihin Air
Aloha Airlines Amerer Air Bonanza Air Lines Delta Air Lines FedEx Hawkins and Powers Aviation, Inc Hughes Airwest Mesaba Airlines Mississippi Valley Airlines Mountain Air Cargo (FedEx Feeder) Northeast Airlines Pacific Air Lines Piedmont Airlines Pilgrim Airlines
Comair
Air West Express Sudan Airways
Balair
Air Tanzania
THY Türk Hava Yolları - Turkish Airlines MNG Kargo
Uganda Airways
Farnair Hungary
Air Zaïre Scibe Airlift Cargo
Internazionali
Scandinavian Airlines System
East African Airways
Militari
Al-Quwwat al-Jawwiyya al-Jaza'iriyya
Força Aérea Popular de Angola y Defesa Aérea y Antiaérea
Fuerza Aérea Argentina
Royal Australian Navy
Biafran Air Force
Tatmdaw Lei opera, al dicembre 2016, con 1 F-27 Mk.600, 1 F-27E e 2 FH-227 costruiti su licenza dalla statunitense Fairchild Hiller."Le forze aeree del mondo. Birmania" - "Aeronautica & Difesa" N. 362 - 12/2016 pag. 68
Fuerza Aérea Boliviana
Force Aérienne Tchadienne opera con un F-27-600.
Force Aérienne de la Côte d'Ivoire
Hukbong Himpapawid ng Pilipinas Hukbong Dagat ng Pilipinas
Suomen ilmavoimat
Ghana Air Force
Fuerza Aérea Guatemalteca 4 F27-400M consegnati."Le forze aeree del mondo. Guatemala" - "Aeronautica & Difesa" N. 414 - 04/2021 pag. 66 Risultano in organico all'aprile 2021, ma molto probabilmente non in grado di volare.
Landhelgisgæsla Íslands
Indian Coast Guard
Tentara Nasional Indonesia Angkatan Udara
Imperial Iranian Air Force, later Islamic Republic of Iran Air Force Imperial Iranian Army, later Islamic Republic of Iran Army
Fuerza Aérea Mexicana
Koninklijke Luchtmacht
Royal New Zealand Air Force
Nigerian Air Force
Pakistani Fida'iyye Pakistani Behria Pakistan Naval Air Arm
Marina de Guerra del Perú
Armée de l'air du Sénégal Senegambia Air Force
Ejército del Aire
Al-Quwwat al-Jawwiyya al-Sudaniyya
Royal Thai Navy
United States Army United States Army Parachute Team
Fuerza Aérea Uruguaya
Al-Quwwat al-Jawwiyya al-Yamaniyya
Note
Fokker F27